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anti sarcomeric myosin heavy chain iia igg mouse antibody supernatant  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti sarcomeric myosin heavy chain iia igg mouse antibody supernatant
    Anti Sarcomeric Myosin Heavy Chain Iia Igg Mouse Antibody Supernatant, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sarcomeric+myosin+heavy+chain/anti-Myosin+heavy+chain+Type+IIA/pm41065612-121-5-15
    Average 96 stars, based on 1084 article reviews
    anti sarcomeric myosin heavy chain iia igg mouse antibody supernatant - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Stem Cells Associated with Adult Skeletal Muscle Can Form Beating Cardiac Tissue In Vitro in Response to Media Containing Heparin, Dexamethasone, Growth Factors and Hydrogen Peroxide.
    Article Snippet: Staining with Pax3/7 antibody (Santa Cruz Biotechnology, Dallas, TX, USA) was performed on cytospun cells, permeabilized for 10 min with 1× PBS containing 0.3% Triton-X and 5% serum. .. For sarcomeric α-actinin, smooth muscle actin, (MilliporeSigma), calponin-1 (InnoGenex, San Ramon, CA, USA) and sarcomeric myosin heavy-chain (MF20; Developmental Studies Hybridoma Bank) antibodies, cells were fixed with cold methanol for 15 min prior to blocking [67]. .. For the detection with MyoD (BD Pharmingen, Franklin Lakes, NJ, USA) and Nkx2.5 (Invitrogen Thermo Fisher Scientific) antibodies, cells were formalin fixed for 30 min prior to a 1 h incubation with 1× PBS with 0.3% Triton-X, and 5% serum.

    Article Title: Stem Cells Associated with Adult Skeletal Muscle Can Form Beating Cardiac Tissue In Vitro in Response to Media Containing Heparin, Dexamethasone, Growth Factors and Hydrogen Peroxide
    Article Snippet: Staining with Pax3/7 antibody (Santa Cruz Biotechnology, Dallas, TX, USA) was performed on cytospun cells, permeabilized for 10 min with 1× PBS containing 0.3% Triton-X and 5% serum. .. For sarcomeric α-actinin, smooth muscle actin, (MilliporeSigma), calponin-1 (InnoGenex, San Ramon, CA, USA) and sarcomeric myosin heavy-chain (MF20; Developmental Studies Hybridoma Bank) antibodies, cells were fixed with cold methanol for 15 min prior to blocking [ ]. .. For the detection with MyoD (BD Pharmingen, Franklin Lakes, NJ, USA) and Nkx2.5 (Invitrogen Thermo Fisher Scientific) antibodies, cells were formalin fixed for 30 min prior to a 1 h incubation with 1× PBS with 0.3% Triton-X, and 5% serum.

    other:

    Article Title: Transplanted microvessels improve pluripotent stem cell-derived cardiomyocyte engraftment and cardiac function after infarction in rats.
    Article Snippet: Human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) offer an unprecedented opportunity to remuscularize infarcted human hearts.. However, studies have shown that most hiPSC-CMs do not survive after transplantation into the ischemic myocardial environment, limiting their regenerative potential and clinical application.. We established a method to improve hiPSC-CM survival by cotransplanting ready-made microvessels obtained from adipose tissue.

    Article Title: Chemotherapeutic drug screening in 3D-Bioengineered human myobundles provides insight into taxane-induced myotoxicities
    Article Snippet: Sarcomeric myosin heavy chain , DSHB , Cat# MF 20; RRID: AB_2147781.

    Staining:

    Article Title: Treatment of volumetric muscle loss in female rats with biomimetic sponges
    Article Snippet: .. The cross-sections were stained with hematoxylin and eosin (H&E; Sigma-Aldrich, St. Louis, MO, USA; Ref: MHS16-500 mL, Ref: HT110116-500 mL respectively), laminin (1:100; ab11575; Abcam; Cambridge, MA, USA; RRID: AB_298179), sarcomeric myosin heavy chain (1:50; MF20; Developmental Studies Hybridoma Bank, Iowa City, IA, USA; RRID: AB_2147781), and collagen 1 (1:100; ab34710; Abcam, Cambridge MA, USA; RRID: AB_731684). .. The appropriate fluorochromeconjugated secondary antibodies (1:100; A11012; RRID: AB_2534079, or A21141 from Invitrogen, Waltham, MA, USA) were used as previously described (Dunn et al., 2022; Haas et al., 2021).



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    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    Developmental Studies Hybridoma Bank sarcomere mhc
    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
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    Developmental Studies Hybridoma Bank sarcomeric myosin heavy chain
    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle <t>(MF20</t> antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
    Sarcomeric Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle (MF20 antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.

    Journal: bioRxiv

    Article Title: A genetically engineered vertebrate animal model of NAA15 -related heart disease uncovers myocardial growth, contractility, and mitochondrial defects

    doi: 10.1101/2025.08.04.668548

    Figure Lengend Snippet: (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle (MF20 antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.

    Article Snippet: The following antibodies were used: anti-GFP (1:1000, chicken anti-GFP, Aves Labs), MF20 anti-sarcomeric myosin heavy chain [1:50, Developmental Studies Hybridoma Bank (DSHB)], anti-Elastin 2b (1:1000), ZN-8 anti-Alcama (1:50, DHSB), CH1 anti-sarcomeric tropomyosin (1:200, DHSB), and anti-dsRed (1:500, Clontech, Cat# 632496).

    Techniques: Control, Double Knockout, Staining, Muscles